EFFECTS OF GILBENCLAMIDE (GLANIL) ON SOME BIOCHEMICAL PARAMETERS OF ALLOXAN INDUCED DIABETIC RATS

Abstract

The effect of gilbenclamide (glanil) was studied on alloxan induced diabetic Sprague dawley rats. The levels of alkaline phosphatase activity, amylase activity, creatinine, total protein, sodium and chloride concentration were determined spectrophotometrically in the serum, kidney, and liver, and blood glucose was determined using glucometer. Establishment of diabetes mellitus in the test animals after eight days was confirmed when the mean fasting blood glucose level in the induced diabetic rats( 291.00± 17.04mg/100ml) was significantly higher ( p<0.05) than that of the control rats (100.20 ±3.06mg/100ml). There was a return to normal fasting blood glucose level of the glanil treated rats (88.25 ± 10.80mg/100ml) after three days treatment. Serum alkaline phosphatase activity of the control, diabetic and glanil treated rats were(3.12 ± 0.44, 6.50 ± 0.50, 8.17 ±2.10) µM/min/ml respectively. There was a significant increase ( p<0.05) in the serum alkaline phosphatase activity of the glanil treated and diabetic rats. Serum amylase activity of the control, diabetic and treated rats were observed as follows (60.49 ±19.23, 140.78 ±8.15,80.87 ±23.34)IU/L respectively. The was a significant increase( p<0.05) in serum amylase of the diabetic rats compared to the treated. Serum creatinine of the control, diabetic and treated rats were (472.28 ±69.13, 619.48 ±76.41,203.23 ±57.72) µmol/L respectively. There was a significant increase (p< 0.05) in the serum creatinine of the diabetic rats compared to the treated. Kidney total protein concentration of the control, diabetic and glanil treated rats were (5.47 ±3.18, 5.94 ±2.45, 5.89 ±2.17) mg/ml respectively. The liver sodium concentration of the control, diabetic, and treated rats were (156.55 ±29.97, 86.18 ±32.80, 82.41 ±20.88) mEq/L respectively. Serum chloride concentration of the control, diabetic, and treated rats were (96.58 ±1.13, 113.27 ±11.63, 70.99 ±16.34) mEq/L respectively. Serum chloride concentration of diabetic rats were significantly increased at (p < 0.05) compared to the treated and control rats. The results showed a reversal to normal fasting blood glucose level of the treated rats and glanil was able to alleviate some of the symptoms of diabetes mellitus. Routine use of glucose level, alkaline phosphatase activity , creatinine concentration, amylase activity, total protein, sodium and chloride concentration measurements be used to monitor diabetic status.